Compensation Toolbox

 

 

This command is found on the Home tab of the ribbon bar, or in the Options menu when using the toolbar and menu display. The Compensation Toolbox allows you to control WinList's interactive N-Color Compensation™ and normalization.  

 

WinList's N-Color Compensation™ can handle any number of fluorochromes and compensate them for signal crossover. In many cases, compensation can be set up automatically, but you can still manually override compensation settings. Compensation can be manually adjusted using 2-parameter dot plots.

 

If you have single-color controls as well as an unstained control file, you can use V-Comp™ to set up your compensation automatically.

 

In order to set up compensation manually, you need a set of single-color control files, one for each fluorochrome you want to compensate. Using Interactive N-Color Compensation demonstrates this approach.

 

WinList also supports reading compensation information directly from FCS files created by many popular cytometers, as well as files containing the FCS 3.1-compliant $SPILLOVER keyword.

 

See also:

Using Interactive N-Color Compensation™

Compensation Toolbox dialog

Compensation Trace Lines