Advice on Compensation dialog

 

This dialog presents information about compensation, normalization, and digital error for the active data file. Review the information in this dialog to determine the characteristics of compensation and the sources of digital error in your system.

 

About Digital Error

When analog data is converted to an integer, an error is introduced known as digital error. Normally this error is quite modest and presents little problem to cytometrists. However, when data is compensated, positive and negative errors are introduced that increase with parameter intensity. The digital error lines in WinList attempt to show the positive envelop of these error distributions for each parameter that is enabled for compensation. If intermediate intensity populations are present that tend to follow the digital error lines, it is possible that they are simply an artifact due to the effects of digital error.