This panel is used to define the expression profile. It is located on the left side of a cell type tab.
Measurement
Enter a short name to use for the profile.
Phenotype
Enter a short phenotype description to use for the profile.
Expression Profile
Choose the type of profile to use for modeling this measurement. See Expression Profile Descriptions for a list of profile types.
Distribution Type
Choose the type of distribution to use for this profile. See Expression Profile Distribution Types for details.
Normal Distribution |
The control definition points define the mean of a normal distribution. |
Inverse Normal |
The profile is the inverse the Normal Distribution. |
Inverse Low Pass |
The profile is the inverse of the Low Pass distribution. |
Low Pass |
The control definition points define a probability "edge". Events below the CDPs are included. |
High Pass |
The control definition points define a probability "edge". Events above the CDPs are included. |
Inverse High Pass |
The profile is the inverse of the High Pass distribution. |
Use for Classifying
Check this option to include this profile in the modeling process.
Use for Enriching
Check this option to include this profile when using the Enrich command.
Exclude from Model
Enable this option to exclude the profile from the modeling process. This is a convenient way to temporarily disable a profile without removing it.
Required
If this option is enabled, the measurement must be included in the FCS file. If it is missing, the modeling process will terminate and the file will not be analyzed.
Estimation Range
This value restricts the estimation routine that adjusts Control Points on the parameter intensity (Y-axis) scale. The value is normalized to a 0 to 100 scale, so a value of 10 means that the estimation routine will consider moving a Control Point as much as 10% up or down if it finds a peak within that range.
Normal Range
This value is the number of Standard Deviations that define the normal range when used with inverse profiles and TriCOMs.
Maximum Transition
This value sets a limit on the amount of transition in a staging profile like a StepUp or StepDown.
Fit Projection
If this option is checked, the modeling process with attempt to fit the projections in order to optimize the fit.
Color
Select a color for this measurement. This color is used in Tri-COMs and overlay plots.
Peak Detection
Peak Fitting Method
Choose the method to use for fitting peaks for this measurement. The default, Both Sides, uses the full peak to estimate position and SD. Steeper side, Dimmer side, and Brighter side use only one side of the peak for the estimates.
Auto Smoothing
Enable this option to automatically determine the best smoothing bandwidth.
Smoothing
Enter a smoothing value. Lower numbers result in less smoothing. Default value is 7.
Minimum Peak Height
The lowest height that is considered a peak.
Minimum State Peak Height
When Fit Projection is enabled, the lowest height that is considered a peak.
Peak Deflection Threshold
The minimum change between a low and high value to consider the segment a peak..
Merge Peaks Below
Peaks below this value will be into one peak.
Peak Separation
If abs(mean1-mean2)/(sd1+sd2) is greater than this threshold, then the peaks are different. (0.10 to 1.00)
Fit Distance
Filters detected peaks where the fitted mean is too far from the peak's location. (1.00 to 4.00)